Twenty-nine of the target genes identified here are associated with HFSCs and hair shaft growth [ 47 - 53 ]. In the present study, we identified axon guidance signaling as the most significant pathway, as it was associated with 62 of the identified target genes. Axon guidance signaling was enriched in multiple signatures, implying that these factors play important roles in driving the large-scale cellular rearrangements necessary for HF formation and orientation [ 68 , 69 ].
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Recent studies have reported that fatty acids can function as ligands for peroxisome proliferator- activated receptors, promoting keratinocyte differentiation [ 70 ]. In this regard, in addition to containing unique miRNAs, exosomes are also characterized by a specific composition of lipids and proteins [ 71 ], and in the present study, we found that the miRNAs expressed in DPC-Exos are related to lipid and fatty acid metabolism signaling pathways.
Exosomes, as important components of paracrine signaling, can mediate the communication between distant cells via the direct transfer of various biomolecules, including miRNAs, from donor to recipient cells [ 72 ]. In the present study, we identified some of the mechanistic roles of these organelles in cellular communication, including their involvement in lipid rafts, the Ras signaling pathway, and endocytosis. The Ras signaling pathway is enriched in exosomes [ 74 ], thereby indicating the mechanistic role of exosomes in cellular activation and that exosomal miRNAs might exert a regulatory function in determining HFSC fate through networking with these cellular communication signaling pathways.
Many paracrine factors and signaling pathways involved in this crosstalk at different stages of the hair cycle have been reported [ 75 , 76 ], and the findings of the present study will facilitate further analysis to determine how DPC-Exos regulate cellular interactions through paracrine mechanisms. Recent research has indicated that miR represses hair neogenesis and HFSC colony formation capacity, and directly inhibits numerous transcription factors upstream the phenotypic keratin genes, including Hoxc13 , Dlx3 , and Foxn1 [ 54 ].
Zhang et al. Our findings provide a platform for further enhancing the intrinsic regeneration of HFSCs, which would provide a valuable basis for the development of clinically viable exosome-based therapeutics. In summary, exosomal miRNAs play a key role in regulating the target genes and signaling pathways that are essential for HFSC proliferation and differentiation, which in turn affect the yield and diameter of cashmere fibers.
Our findings also provide new insights on how to modify the direction HFSC differentiation for tissue engineering purposes. Determination of hair follicle characteristics, density and activity of Iranian cashmere goat breeds. Small Ruminant Research.
Asian-Australasian Journal of Animal Sciences. Cyclical fleece growth in cashmere goats. Australian Journal of Agricultural Research. Blanpain C, Fuchs E. Plasticity of epithelial stem cells in tissue regeneration. Lichen planopilaris is characterized by immune privilege collapse of the hair follicle's epithelial stem cell niche. The Journal of pathology. Alopecia areata. New England Journal of Medicine. Hair follicles' transit-amplifying cells govern concurrent dermal adipocyte production through Sonic Hedgehog.
Dermal cell populations show variable competence in epidermal cell support: stimulatory effects of hair papilla cells. Journal of cell science. Lef1 contributes to the differentiation of bulge stem cells by nuclear translocation and cross-talk with the Notch signaling pathway.
International journal of medical sciences. Regulation of hair follicle development by exosomes derived from dermal papilla cells. Biochemical and Biophysical Research Communications. Biochemical and biophysical research communications. Nature cell biology. Transfer of microRNAs by embryonic stem cell microvesicles. PloS one. Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages.
Journal of translational medicine.
Altered microRNA expression profile in exosomes during osteogenic differentiation of human bone marrow-derived mesenchymal stem cells. Micro-RNA controls hair cycle-associated changes in gene expression programs of the skin and hair follicle. MicroRNA controls skin and hair follicle development by modulating the activity of the Wnt pathway.
J Cell Biol. BMC genomics.
Integrative analysis reveals ncRNA-mediated molecular regulatory network driving secondary hair follicle regression in cashmere goats. Arterioscler Thromb Vasc Biol. Scientific Reports. Isolation and characterization of hair follicle stem cells from Arbas Cashmere goat. Bickenbach JR, Chism E.
Selection and extended growth of murine epidermal stem cells in culture. Experimental Cell Research. Fibroblast growth factor 5-short FGF5s inhibits the activity of FGF5 in primary and secondary hair follicle dermal papilla cells of cashmere goats. Effect of miRb on dermal papilla cells of goat secondary hair follicle. Electronic Journal of Biotechnology.
Transcriptome sequencing reveals differences between primary and secondary hair follicle-derived dermal papilla cells of the Cashmere goat Capra hircus.
Journal of Biological Chemistry. Martin M. Cutadapt removes adapter sequences from high-throughput sequencing reads. Embnet Journal. Li H, Durbin R. Storey JD. The positive false discovery rate: a Bayesian interpretation and the q-value. Annals of Statistics. A pattern-based method for the identification of MicroRNA binding sites and their corresponding heteroduplexes. The role of site accessibility in microRNA target recognition.
Nature Genetics. Gene ontology analysis for RNA-seq: accounting for selection bias. Genome Biology. Stem cells in the hair follicle bulge contribute to wound repair but not to homeostasis of the epidermis. Nature medicine. An autologous epidermal equivalent tissue-engineered from follicular outer root sheath keratinocytes is as effective as split-thickness skin autograft in recalcitrant vascular leg ulcers.
Shim JH. Extracellular vesicles derived from MSCs activates dermal papilla cell in vitro and promotes hair follicle conversion from telogen to anagen in mice. Sci Rep. Label-retaining cells reside in the bulge area of pilosebaceous unit: implications for follicular stem cells, hair cycle, and skin carcinogenesis. Hair follicle stem cells in the lower bulge form the secondary germ, a biochemically distinct but functionally equivalent progenitor cell population, at the termination of catagen. Getting to the Core of the Dermal Papilla. Journal of Investigative Dermatology. Nat Cell Biol. At about 12 weeks, it is believed that specific signals from the dermis are released- signaling for the induction hair follicle formation  .
Through secreted proteins such as Sonic Hedgehog, the placode continues to proliferate and enclose the dermal condensate. This eventually forms a deep, club-shaped hair bud, with an invaginated dermal papillae . These dermal papillae are rapidly infiltrated by blood vessels and nerve endings. In this stage, it is also believed that the polarity of the hair follicle the angle at which hair-follicles grow in relation to skin and the architecture of the hair follicle itself straight hair, wavy hair, etc is regulated in part by Sonic Hedgehog and TGF-a signaling respectively .
The epithelial cells within the hair bulb, begin to differentiate into the germinal matrix — which grow, proliferate and keratinise to form the hair shaft and internal root sheet . Other epithelial cells outside of the hair bud, form the external hair sheeth. Mesodermal cells of the dermis that surround the invaginating hair follicle form the dermal root sheeth and the arrecrtor pili muscles for hairs.
Proteins such as Notch1 are believed to help regulate the phenotype of keratinocytes as they differentiate . Lanugo Hair . Together, fingernails and toenails are modifications of the epidermis which are derived from the same embryonic origin, the ectoderm. The functions of vernix caseosa include:. There are two major kinds of sweat glands present in humans, both of which develop from downgrowths of the epidermis into the underlying dermis. Sweat glands have been histologically identified in studies from week 21 of development. They begin as cellular buds  , which proliferate as solid, cylindrical down growths into mesenchyme.
Central cells degenerate to form a lumen, while the terminal region coils to eventually form the body of the gland. As fetal development continues, peripheral cells eventually differentiate into secretory and myoepithelial cells . The ectoderm and the associated underlying layer of neural crest cells, are the origin for teeth development. Teeth have the main function of processing food, but also frequently serve other functions in relation to defense, display of dominance and phonetic articulation in humans . Also, as opposed to the earlier Lamina stage, in the Bud stage of tooth development, the odontogenic potential is lost from the epithelium and teeth may now only grow within the ectomesenchyme .
Also, during this stage, the cells differentiate in situ, and the crown takes its final shape . The dental papilla, which forms from the neural crest cells that underlie the enamel organs  eventually give rise to the dental pulp and odontoblasts- which produce predentin and dentin, in the adult body. With further development and immediate predentine deposition  , these cells then take a columnar shape and differentiate into ameloblasts that start synthesizing and depositing enamel.
Knowledge of the Integumentary expands in conjunction with technological developments that allow observation of microscopic structures.
Historically animal models have been used to map the stages in the development of the fetal integumentary system. The study of hair follicle development was primarily motivated by the need to understand abnormalities in development.
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Determined that the hair fibre finally breaks through the epidermis and appres about the skin at around weeks of development. The lanugo foetal hair is extremely fine with no medulla and a tip free of pigment. Microscopy and staining techniques have been essential tools in study of nail development, anatomy and physiology. Thus, as such technology advances scientists have been able to uncover the morphological details of the nail and understand the changes that occur during nail development.
A review of the history allows us to recognize the major contributor to this field. History of research on nail development also reveals the interesting debate on the origins of keratinized cells which migrate to form the nail palate in weeks of fetal development. The current understanding of fetal nail development is a summation contributions of many researches in the international scientific community. Today research continues, technology allows exploration beyond macroscopic and microscopic morphological changes in the fetal nail.
Now the aim is to understand the molecular signalling with in the epidermal cells which drives this process. Aplasia cutis congenita ACC is a rare skin abnormality, characterised by the absence of all layers of the skin. In severe cases, the defect can go as deep as the bone or the dura. Other sites of ACC include the skin of the limb regions. The damage to the vertex is suggested to be the result of the biomechanical stretch at this area when the fetal brain is growing.
Presently, ACC is managed via conservative treatments or surgical treatments. Conservative treatments refer to basic wound treatments and preventing infection with the use dressings and antibiotics. Surgical treatments, specifically scalp reconstruction procedures, aim to reconstruct the damage to the skin through skin grafts, local scalp flaps, and pericardial scalp flaps. Large defects are often treated using surgical treatments. Dystrophic Epidermolysis Bullosa DEB , a type of epidermolysis bullosa, is a genetic disease of the skin, usually present at birth or at an early age.
Currently, around , - , people are affected with the disease . It is characterised by the fragility of the skin  , where it blisters upon minimal trauma and scars  , usually at the extremities . It is caused by a mutation in collagen VII gene COL7A1   , which is responsible for the the formation of anchoring fibrils . In some cases, even teeth and nails are affected. Teeth of patients with DEB have enamel defects and when combined with poor oral hygiene, it may lead to decay. Nails of DEB patients are often dystrophic and will eventually be lost.
There are currently no known cures for DEB; however there are techniques to manage the clinical manifestations of the disease, which include:. One study is currently exploring the potential of protein therapy as a treatment for DEB. Alopecia areata AA is an abnormality of the hair affecting anagen hair follicles, characterised by well-demarcated patches of hair loss. While others lose all of their body hair, this is called alopecia universalis. There is an abnormality with the genes related to the immune system and to the hair follicles.
And histopathology shows signs of lymphatic infiltration of the hair follicles and the loss of these scalp lymphocytes allow hair follicles to recover. There is currently no cure for AA. There are several treatments to combat AA but none of these have led to remission of the disease, the most effective being corticosteroids and topical immunotherapy. Transepidermal drug delivery TED is a new treatment that functions by creating micro-channels in the epidermis. By doing so, drug delivery to the skin is improved.
This treatment was highly effective and had lower rates of side effects, e. Congenital ichthyosis is an autosomal recessive disease of the skin, characterised by visible and excessive scaling of the skin and hyperkeratosis, i. Hypohidrotic ectodermal dysplasia HED is the most of all ectodermal dysplasias, caused by an abnormality in the development of ectodermal tissues, which inlude skin, hair, teeth, sweat glands, and nails.
HED is mainly characterised by hypohidrosis due to the lack of sweat glands in the skin, which could lead to hyperpyrexia and sometimes death. The mutations of this gene results in the poor sweating ability or none at all in a person. The effects of this abnormality is usually more severe in males than in females. There is currently no pharmacological therapies for HED but there are methods applied to prevent the disease from aggravating. Neonates with HED are placed in incubators and monitored to prevent them from overheating.
Management of this disease gets easier as the patient ages. Adults with HED can control their thermoregulation by staying in cool environments or drinking cold drinks to lower the body temperature. Currently, there are studies that aim to find a cure for this abnormality, e. Navigation Main page. Site map. Site updates. Recent changes. New images. Teaching Medicine. BGD2 Tutorial. Med Projects. Sci Projects. In conclusion, we show here that adipose tissue provides for an excellent source of endothelial cells for tissue engineering purposes, since they are readily available, and easily isolated and amplified.
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This study assessed the contribution of subcutaneous adipose tissue to exacerbated wound inflammatory conditions. Of importance, the critical regulator of adipocyte maturation, PPAR-gamma, was merely expressed in the final phase of in-vitro induced adipocyte differentiation from 3T3-L1 pre-adipocytes. The inhibitory effect of PPAR-gamma activation on CXCL2 release appeared to be a general anti-inflammatory effect in mature adipocytes, as cytokine-induced cyclooxygenase Cox -2 was simultaneously repressed by rosiglitazone.
In accordance with these findings, oral administration of rosiglitazone to wounded obese mice significantly changed subcutaneous adipocyte morphology, reduced wound CXCL2 and Cox-2 expression and improved tissue regeneration. Thus, our data suggest that PPAR-gamma might provide a target to suppress inflammatory signals from mature adipocytes, which add to the prolonged wound inflammation observed in diabetes-obesity conditions.
While macrophages can be divided into the classically-activated M1 and the alternatively-activated M2 phenotype, ASCs are characterized by the expression of specific stem cell markers. METHODS: In the present study, we have investigated the expression of common macrophage polarization and stem cell markers in acutely inflamed adipose tissue.
Subcutaneous adipose tissue adjacent to acutely inflamed wounds of 20 patients and 20 healthy subjects were harvested and underwent qPCR and flow cytometry analysis. By flow cytometry, a significant shift of adipose tissue macrophage populations towards the M1 phenotype was confirmed. NaN dermis Perivascular stem cells PSCs are the natural ancestors of mesenchymal stem cells MSCs and are the stem cells responsible for homeostasis and repair in vivo.
Prospectively identified and isolated PSCs have demonstrated increased plasticity and osteogenic potential. Cells from the infrapatellar fat pad IFP have demonstrated increased chondrogenic potential compared with those from subcutaneous fat. Pericytes and adventitial cells were isolated from the stromal vascular fraction 3. The mean numbers of pericytes and adventitial cells isolated were 4. Differentiation was confirmed using histochemical stains and genetic expression.
The IFP was a significantly better source of chondrogenic stem cells compared with bone marrow. Stem Cells Translational Medicine ; NaN subcutis Perivascular stem cells PSCs are the natural ancestors of mesenchymal stem cells MSCs and are the stem cells responsible for homeostasis and repair in vivo. Different regions of the normal skin of three different ages newborn, half-year-old and adult of yaks were harvested for histological study and thickness measurement. Biopsy samples were taken from the scapula regions of the skin from the same five approximately 1-year-old yaks during the hair cycle telogen, anagen and catagen.
The IPP6. The unique features included prominent cutaneous vascular plexuses, underdeveloped sweat glands, a large number of nasolabial glands in the nasolabial plate, and hair follicle groups composed of one primary follicle and several secondary follicles. The skin, epidermis and dermis thickness did vary significantly between different body regions and different ages. The thickness of the skin, epidermis and dermis increased from newborn to adult in yaks. Yak skin thickness decreased from dorsally to ventrally on the trunk.
The skin on the lateral surface was thicker than the skin on the medial surface on the limbs. The expression of HSP27 mRNA and protein in the anagen stage was the highest, followed by the catagen stage, and the expression in the telogen stage was the lowest. The expression of HSP70 mRNA and protein in the telogen stage was the highest, followed by the anagen stage, and the expression in the catagen stage was the lowest.
The expression of HSP90 mRNA and protein in the anagen stage was the highest, followed by the telogen stage, and the expression in the catagen stage was the lowest.
HSPs were mainly expressed in the outer root sheath of hair follicle during the hair cycle, also expressed in epidermis, sebaceous gland and sweat gland in the skin of Yak. The expression of CGI mRNA in the anagen stage was the highest, followed by the catagen stage, and the expression in the telogen stage was the lowest. The expression of KDF1 mRNA in the telogen stage was the highest, followed by the catagen stage, and the expression in the anagen stage was the lowest. MEANING: In this study, we examined and fully described the histology of normal skin in Yak and measured the skin thickness of different ages and different regions in Yak.
These data may be useful to better understand and appreciate the adaptability features of yak skin. Our investigation reports the expression patterns of HSPs in yak skin for the first time. The different expression pattern of HSPs during the hair cycle suggests they may play different roles in yak hair follicle biology. However, the regulation mechanism is still not elucidated completely at molecular level. In the present study, we comparatively analyzed transcriptome of subcutaneous adipose tissue between Wagyu and Holstein cattle with a significant difference in fat deposition to identify key genes associated with fat metabolism and adipogenesis by high-throughput RNA-seq technology.
A total of 59,, and 69,, high quality reads were generated, respectively. With further analysis, differentially expressed genes were identified. Moreover, potential alternative splicing events and single nucleotide polymorphisms SNPs were also identified. In summary, we comprehensively analyzed and discussed the transcriptome of subcutaneous adipose tissue of Wagyu and Holstein cattle, which might provide a theoretical basis for better understanding molecular mechanism of fat metabolism and deposition in beef cattle.
Here we examine the role of cell proliferation regulator Kruppel-like factor 5 Klf5 in adult mouse CE homeostasis. Normal chow-fed ternary transgenic siblings served as controls. In this study, we assessed the effects of Pinus densiflora extract PDE on photoaging and investigated its mechanism of action in human skin fibroblast Hs68 cells after UVB exposure using real-time polymerase chain reaction, Western blot analysis, and enzymatic activity assays. PDE exhibited an antioxidant activity and inhibited elastase activities in vitro. Moreover, PDE prevented the decrease of type I procollagen mediated by exposure to UVB irradiation, an effect that is linked to the upregulation and downregulation of Smad3 and Smad7, respectively.
NaN dermis PURPOSE: We aimed to investigate residual adipose tissue with whole-body magnetic resonance imaging to differentiate between subtypes of lipodystrophy. CGL4 caused by mutations in the PTRF gene was characterized with well-preserved retro-orbital and bone marrow fat in the absence of any visible residual adipose tissue in other areas. No residual adipose tissue was observed in AGL. Despite loss of subcutaneous fat, periarticular adipose tissue was preserved in the lower limbs of patients with FPLD.
Retro-orbital adipose tissue was surprisingly preserved in APL, although they lacked head and neck fat. Our results suggest that residual adipose tissue characteristics can help distinguish different subtypes of lipodystrophy. This study examined the effects of chemical chaperone treatment sodium 4-phenylbutyrate on fibroblast cell lines derived from men with missense mutations. Methods: Dermal fibroblast cultures were established from 2 affected men and 3 normals. Proliferation rates were examined, the collagen IV alpha5 chain localized with immunostaining, and levels of the intra- and extracellular chains quantitated with an in-house enzyme-linked immunosorbent assay.
Measurements were repeated after 48 hours of incubation with 10 mM sodium 4-phenylbutyrate acid. Whether these actions delay end-stage renal failure in men with X-linked Alport syndrome and missense mutations will only be determined with clinical trials. NaN dermis Background: Familial partial lipodystrophy of the Dunnigan type is one of the most common inherited lipodystrophies variables.
These individuals have important metabolic disorders that cause predisposition to various diseases. In this study we aimed to demonstrate the relation between the metabolic abnormalities, inflammatory profile and the expression of genes involved in the activation of the endoplasmic reticulum stress ERS in subjects with FPLD. Patients were fasted for 12 h before blood collection for measurement of HbA1c, glucose, insulin, lipids and inflammatory markers. Subcutaneous adipose tissue was collected by puncture aspiration of submental region during ambulatorial surgical aesthetic procedure. We found that Conclusion: Individuals with FPLD besides having typical dysfunctions of metabolic syndrome, show a hyperactivation of ERS associated with increased systemic inflammatory profile, which together may explain the complex clinical aspect of this diseases.
NaN subcutis Background: Familial partial lipodystrophy of the Dunnigan type is one of the most common inherited lipodystrophies variables. The importance of this study stems from the fact that riverine buffaloes are major dairy species of Indian sub-continent and the information generated here will be of great interest to the investigators engaged in functional genomic studies of this important livestock species. In addition to overall analysis, tissue wise evaluation of expression stability of individual RG was also performed. Three different algorithms provided in geNorm, NormFinder and BestKeeper softwares were used to evaluate the stability of 10 potential reference genes from different functional classes.
The M-value given by geNorm ranged from 0. This manuscript thus provide useful information on panel of reference genes that could be helpful for researchers conducting functional genomic studies in riverine buffaloes. Though innovative in theory, a major hindrance to the practical application of this treatment is that the hCPCs of elderly patients, who are most susceptible to myocardial disease, are senescent and prone to cell death. Rejuvenating hCPCs from elderly patients may help overcome this obstacle, and can be accomplished by reversing entry into the cellular stage of senescence.
Our results suggest that genetic knockdown of p16 INK4A may play a significant role in inducing antioxidant effects and extending lifespan of aging hCPCs.
This genetic modification may enhance the effectiveness of autologous hCPC therapy for repair of infarcted myocardium. It remains unclear whether beneficial effects of weight loss are related to AT inflammation. We aimed to assess the effect of weight loss during low-calorie diet on insulin sensitivity, AT expression of genes associated with inflammation in young subjects with obesity. Furthermore, we estimated the effects of immunomodulatory 1, 3 1, 6 -beta-glucan BG on the above parameters.
Twelve-week dietary intervention was applied, with randomization to receive or not mg BG daily. Euglycemic hyperinsulinemic clamp, subcutaneous AT biopsy were performed before and after the program. Twenty normal-weight subjects, examined at baseline, served as a control group. Forty obese subjects completed dietary intervention program, which resulted in BG addition had no effect on any of the parameters studied. BG does not have effects during dietary intervention. In the present study, the inflammatory activity of the IFP, the main adipose structure within the stifle joint, was thoroughly investigated to evaluate its potential impact in the pathogenesis of this common disease of our canine companions.
Characterization of the immune cell composition was ascertained by fluorescence activated cell sorting. T cells and macrophages CD14 were significantly increased compared to healthy controls or corresponding ScAT. TNFalpha was only increased on gene expression. To determine the extent of this possible inter-relationship, further studies need to be undertaken. Toll-like receptors TLRs are integral in the initiation of inflammatory signaling. Therefore, we evaluated the effect of TLR-deficiency on dry eye-related ocular surface damage and inflammation using a mouse model of experimental dry eye EDE.
Tear production was measured by phenol red thread test and ocular surface damage assessed with fluorescein staining. Corneal homogenates were obtained for matrix metalloproteinase MMP and cytokine expression analysis by Luminex assay and quantitative PCR. In addition, whole eyes and eyelids were dissected and goblet cells and Meibomian glands were imaged, respectively.
MyDdeficiency also resulted in decreased corneal cytokine levels. Conclusions: These studies demonstrate the importance of TLR signaling in dry eye development. NaN dermis The transcriptional response of cells exposed to proton radiation is not equivalent to the response induced by traditional photon beams.
Changes in cellular signalling is most commonly studied using the method Quantitative polymerase chain reaction qPCR. Stable reference genes must be used to accurately quantify target transcript expression. The study aim was to identify suitable reference genes for normalisation of gene expression levels in normal dermal fibroblasts irradiated with either proton or photon beams. The online tool RefFinder was used to analyse and identify the most stably expressed genes from a panel of 22 gene candidates.
To assess the reliability of the identified reference genes, a selection of the most and least stable reference genes was used to normalise target transcripts of interest.
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Fold change levels varied considerably depending on the used reference gene. NaN dermis In this study, we investigated the anti-microbial, anti-inflammatory, and anti-lipogenic effects of hemp Cannabis sativa L. Moreover, P. In addition, the transactivation of AP-1 induced by P.